ABSTRACT
BACKGROUND:There are many treatment methods for infected bone defects,but there is no first stage treatment method,which has the characteristics of sustained-release,anti-inflammation,osteogenic activity,bone conduction and degradable absorption.OBJECTIVE:To investigate the research progress of bone tissue engineering technique in the treatment of infected bone defects.METHODS:A computer-based retrieval of PubMed,WanFang and CNKI databases was conducted for the articles published from 2000 to 2016,concerning the basic and clinical research on the local application of antibiotics;basic research on infected bone defects with sustained-release of antibiotics;basic studies on the source of seed cells and the osteogenic mechanism;and scaffold materials.A total of 55 eligible articles were included for result analysis.RESULTS AND CONCLUSION:Topical application of antibiotics exhibits different effects in the treatment of osteomyelitis.The development of bone tissue engineering has brought new hope for the treatment of bone defects,and in the meanwhile,selection of excellent seed cells has become a hot and difficult research.A rational combination of antibiotics,seed cells and scaffold materials may provide a new treatment strategy for infected bone defects.
ABSTRACT
Objective To develop and optimize a new method to extract miRNAs from plasma.Methods miRNAs were extracted from plasma by mixing it with the extraction solution that contained surfactant and by heating .Then the ribonuclease inhibitor was added into the extraction to prevent RNAs from degradation .The expression level of each miRNA was detected by real-time quantitative PCR in oder to evaluate the feasibility of this method .Results A method which extracted miRNAs from plamsa in just one step was established .The specificity , reproducibility and stability of this method have been demonstrated by real-time quantitative PCR .Conclusion The one-step method is simple , inexpensive , and plasma-saving.It seems like a new method for clinical examination of miRNAs from plasma .
ABSTRACT
Senescence is one of the most important phenomena in cells' life. It is hold by one of hypothesis for cell senescence that residue DNA damages of a cell will accelerate its senescence.The normal function of surveillance and repair system for DNA damage is highly related with the senescence regulation of a cell. As a result, research of senescence regulation role of enzymes related for surveillance and repair of DNA damage, such as PARP, DNA-PK, ATM, p53, etc., will discover the inner relation between stress response of cell to DNA damage, regulation of DNA damage repair and cell senescence. That may be helpful for research of anti-aging and treatment of tumor by regulation of senescence of tumor cells.
ABSTRACT
Regulation of gene expression is one of the most importa nt responses of cells to DNA damage induced by radiation. A novel expressed seq u ence tag (EST) fragment had been cloned from human embryo lung cells induced by 50cGy radiation and named RIG1. To clone the full-length cDNA of RIG1, a non-c loned cDNA library of human embryo lung cells induced by low dose irradiation ha d been established. This library was used as template in enchanced nest RACE PCR and biotin-labeled probe was used for further purification. The 3′ flanking s equence of this EST was cloned and sequenced with this set of technology. It was illuminated by homology analysis that this 3′ flanking sequence and the origin al EST are well aligned with a BAC clone of 20th chromosome and the predic ted exons sequence of this chromosome is well consisten ce with the real EST. Thus the RIG1 can be roughly located in 20th chromos ome. By use of the exons sequence predicted from chromosome sequence by GENSCA N, full-length of RIG1 gene has been cloned. Chromosome location of RIG1 gene i s further determined by this successful verification of Bioinformatics predictio n by experiment. By the same step, genome sequence of RIG1 has been determined. Therefore,by the combined use of Bioinformatics analysis，the full-length cDNA sequence and genome sequence of RIG1 gene are obtained and the predicted protei n sequence is determined.